PNPase knockout results in mtDNA loss and an altered metabolic gene expression program
Published in PLoS ONE, 2018
Recommended citation: Shimada E, Ahsan FM, Nili M, Huang D, Atamdede S, TeSlaa T, Case D, Yu X, Gregory BD, Perrin BJ, Koehler CM, Teitell MA. (2018). "PNPase knockout results in mtDNA loss and an altered metabolic gene expression program." PLoS ONE. 13(7), e0200925. DOI: https://doi.org/10.1371/journal.pone.0200925 http://fahsan.github.io/files/ShimadaAhsan-PLoSOne-2018.pdf
Polynucleotide phosphorylase (PNPase) is an essential mitochondria-localized exoribonu- clease implicated in multiple biological processes and human disorders. To reveal role(s) for PNPase in mitochondria, we established PNPase knockout (PKO) systems by first shifting culture conditions to enable cell growth with defective respiration. Interestingly, PKO estab- lished in mouse embryonic fibroblasts (MEFs) resulted in the loss of mitochondrial DNA (mtDNA).
Recommended citation: Shimada E, Ahsan FM, Nili M, Huang D, Atamdede S, TeSlaa T, Case D, Yu X, Gregory BD, Perrin BJ, Koehler CM, Teitell MA. (2018). “PNPase knockout results in mtDNA loss and an altered metabolic gene expression program.” PLoS ONE. 13(7), e0200925. DOI: https://doi.org/10.1371/journal.pone.0200925